Journal: Journal of Neuroinflammation

Article Title: Influenza A virus infection disrupts oligodendrocyte homeostasis and alters the myelin lipidome in the adult mouse

doi: 10.1186/s12974-023-02862-2

Figure Lengend Snippet: Influenza infection does not affect OL survival but increases marker of OL stress in the mPFC. a Representative immunohistochemical staining of mPFC tissue of PBS- and IAV-inoculated mice with anti-APC (CC1 clone), SOX10, Iba-1, and GFAP at day 8 p.i. b , c Number of SOX10 + APC + and SOX10 + APC − cells per mm 2 mPFC ( n = 3–5), and number of Iba-1 + and GFAP + cells per mm 2 mPFC ( n = 6–8) of PBS- and IAV-inoculated mice at day 8 p.i. Flow cytometry was performed on brains of PBS- and IAV-inoculated mice ( n = 5) at day 8 p.i. to determine percentage of myelin debris (SOX10 − MAG + ), immature OLs (SOX10 + MAG − ), and mature OLs (SOX10 + MAG + ) ( d , e ), as well as OL protein expression of immature OLs, mature OLs, and myelin debris ( f – i ). Mean fluorescent intensity (MFI) of SOX10 protein expression of immature OLs ( f ) and mature OLs ( g ), MFI of MAG and PLP protein expression of mature OLs ( h ) and myelin debris ( i ). Data analyzed by Student’s t -test and presented as mean ± SEM. P-value * < 0.05, *** < 0.001

Article Snippet: Cells were fixed and permeabilized overnight at 4 °C with Foxp3 Transcription Factor Fix/Perm Buffer (Invitrogen #00-5523) and then stained with Alexa Flour 488 conjugated mouse anti-MAG (Clone 513; Sigma #MAB1567A4) as well as unconjugated rabbit anti-Sox10 (Clone SP267; Abcam #ab227680) and rat anti-PLP (Clone AA3; Sigma #MABN2620) for 1 h on ice.

Techniques: Infection, Marker, Immunohistochemical staining, Staining, Flow Cytometry, Expressing

Journal: iScience

Article Title: Respiratory infection with influenza A virus delays remyelination and alters oligodendrocyte metabolism

doi: 10.1016/j.isci.2024.110464

Figure Lengend Snippet:

Article Snippet: anti-PLP (AA3; rat monoclonal) , Millipore Sigma , Cat# MABN2620-100UG; RRID: AB_3083553.

Techniques: Virus, Recombinant, Gene Expression, Software

Journal: Annals of neurology

Article Title: Intrinsic and Extrinsic Mechanisms of Thalamic Pathology in Multiple Sclerosis

doi: 10.1002/ana.25743

Figure Lengend Snippet: Representative case of a subject with grossly apparent hyperpigmented thalamic lesions on a 1 cm fixed coronal slice (A); enlarged lesion shown below. Both ovoid (B) and subependymal (C) lesions are noted using PLP immunohistochemistry for myelin; asterisk (*) denotes location of vessel. Magnetic resonance imaging (MRI) coronal images, with thalamus depicted with a white outline, (D) depict fluid-attenuated inversion recovery (FLAIR) and T1-Magnetization Prepared Rapid Acquisition of Gradient Echo (MPRAGE) sequences as well as ovoid (blue) and subependymal (Subep.; green) manually segmented lesions. The heart-shaped hyperpigmented lesion straddling the dorsomedial and ventrolateral nuclei is indicated with an arrow and an enlarged inset of the lesion is below (A). The lesion is demyelinated (B) and labeled as ovoid/perivascular (“Ovoid”) on MRI T1 MPRAGE (D). Subependymal lesions (C) were not identified on gross examination (A) and subtle on MRI (D; labeled “Subep.”). PV, perivascular; PLP, proteolipid protein.

Article Snippet: Primary antibodies included: proteolipid protein (PLP; AA3 rat clone, a gift from Wendy Macklin, University of Denver, CO, 1:250 dilution) for myelin, HuR (Santa Cruz Biotechnology, Dallas, TX, mouse IgG, SC-5261, 3A2 clone, 1:500 dilution) for neurons, major histocompatibility complex class II (MHCII, HLA-DR CR3/43, Dako, Santa Clara, CA, mouse monoclonal, M0775, 1:500 dilution) for activated microglia, and SMI31 (mouse IgG1k, Biolegend, San Diego, CA, 801601, 1:2500 dilution) and SMI32 (mouse monoclonal, Biolegend, 801701, 1:2500 dilution) for axons and dendrites.

Techniques: Immunohistochemistry, Magnetic Resonance Imaging, Labeling

Journal: Annals of neurology

Article Title: Intrinsic and Extrinsic Mechanisms of Thalamic Pathology in Multiple Sclerosis

doi: 10.1002/ana.25743

Figure Lengend Snippet: Patterns of thalamic lesions characterized by immunohistochemistochemical staining for myelin (PLP) and activated microglia/macrophages (MHCII). Lesions top to bottom: chronic inactive (hypocellular center with asterisk*), chronic active (rim of hypercellularity; vessels labeled with “V”), active (hypercellular), and subependymal (Subep.) demyelination, areas of perivascular inflammation without demyelination (vessels labeled “>”). Scale bars are 3 mm for all images except for “PV inflammation” (600 μm). MHC, major histocompatibility complex; NAGM, normal appearing grey matter; PLP, proteolipid protein; PV, perivascular.

Article Snippet: Primary antibodies included: proteolipid protein (PLP; AA3 rat clone, a gift from Wendy Macklin, University of Denver, CO, 1:250 dilution) for myelin, HuR (Santa Cruz Biotechnology, Dallas, TX, mouse IgG, SC-5261, 3A2 clone, 1:500 dilution) for neurons, major histocompatibility complex class II (MHCII, HLA-DR CR3/43, Dako, Santa Clara, CA, mouse monoclonal, M0775, 1:500 dilution) for activated microglia, and SMI31 (mouse IgG1k, Biolegend, San Diego, CA, 801601, 1:2500 dilution) and SMI32 (mouse monoclonal, Biolegend, 801701, 1:2500 dilution) for axons and dendrites.

Techniques: Staining, Labeling, Immunopeptidomics